Antidermatophytic effect of Stachys schtschegleevii extract on Microsporum canis(IBRC-M ۳۰۲۱۵)

Background and Aim : Microsporum canis is a zoophilic dermatophyte that causes dermatophytosis. This is a prevalent disease and should be treated properly. There are many reports of resistance to antifungal drugs. On the other hand, these drugs have many side effects so medicinal plants can be appropriate alternatives . Stachys schtschegleevii is a medicinal plant that is used for treatment of respiratory and urinary system infections, Rheumatism and other inflammatory diseases. S. schtschegleevii also has antimicrobial, antioxidant and anti-cancer effects. The aim of this study is to evaluate the effect of Stachys schtschegleevii extract on Microsporum canis. Methods : M. canis (IBRC-M ۳۰۲۱۵) prepared from microorganisms bank of Iranian Biological Resource Center. Then cultured in SCC and incubated in ۲۸°c for ۷-۱۴ days. After preparation of S. schtschegleevii, the extraction carried out using soxhlet method. The antifungal effect of S. schtschegleevii extract was measured by disc diffusion method, MIC۸۰ (Microdilution method) and MFC.Results : According to the results, the inhibitory zone of S. schtschegleevii extract was ۱۹±۱ mm which was not significantly different from that of Griseofulvin ( p?۰.۰۵). The Minimum Inhibitory Concentration ( MIC۸۰) of S. schtschegleevii extract was ۵±۰.۵ mg/ml. The Minimum Fungicidal Concentration (MFC) of S. schtschegleevii extract was ۱۰±۰.۸۵ mg/ml Which was not significantly different from that of Griseofulvin, Nystatin and Terbinafine (p?۰.۰۵). Conclusion : Overall, the results of this research showed that S. schtschegleevii extract has a significant antidermatophytic effect on Microsporum canis.

کلیدواژه ها:

dermatophytosis ، Microsporum canis ، Stachys schtschegleevii