Antidermatophyte effect of zinc nanoparticle on Epidermophyton floccosum(IBRC-M ۳۰۲۲۳)

Background and Aim : In the last decades Nanotechnology development in the Science and Technology. Metal nanoparticles (NPs) are used in many industrial applications. Among the different metals, zinc attracts more attention. Zinc nanoparticles are one of the most widely used mineral nanoparticles that are considered by researchers due to their suitable physical and chemical properties that used in the cosmetics industry, especially sunscreens and food industry, their antimicrobial properties have also been proven. In this study, we investigated the antifungal effect of Zinc nanoparticles . Dermatophytosis is one of the most common mycosis that caused by Dermatophytes. Dermatophytes colonized on the keratin tissues and cause infections in skin, hair, and nails. Now a day drug resistance is one of the main problems and researchers are looking for alternatives, one of the effective alternatives is Nanoparticles so in this study, we used zinc nanoparticles and effect of zinc nanoparticles on Epidermophyton floccosum was investigatedMethods : Epidermophyton floccosum (IBRC-M ۳۰۲۲۳) was prepared from Iranian biological Resource Center and cultured in SCC and incubated in ۲۸ ºC for ۷-۱۴ day. Zinc oxide nanoparticles was prepared from Nano Nasb Pars Company, the size of them was ۲۰-۵۰ nm. Antifungal effect of them was measured by disc diffusion method, Minimum Inhibitory Concentration (MIC۸۰) by Microdilution method, and Minimum Fungicidal Concentration (MFC)was measured. Results : The results showed that the inhibition zone of the zinc nanoparticles were ۲۱±۰.۵ mm which is not significantly different from that Griseofulvin (p≤۰.۰۵) and The concentration of MIC۸۰ has been ۸۰±۱ µg/ml and the concentration of zinc nanoparticles obtained as an MFC has been ۹۰±۱ µg/ml which is not significantly different from that of Griseofulvin, Nystatin and Terbinafine (p≤۰.۰۵). Conclusion : According to the results, the zinc nanoparticles had antifungal effect potential on Epidermophyton floccosum. As a result, the zinc nanoparticles are a very suitable and safe substitute for the treatment of fungal diseases such as dermatophytosis.

کلیدواژه ها:

Epidermophyton floccosum ، Zinc Nanoparticles ، Dermatophyte