ZnO/GQDs -NPs nanocomposites enhances the Antibacterial Activity of Dermatocarpon miniatum against Methicillin-resistant Staphylococcus aureus isolated from burn wounds

Background and Aim : Over the last few years, the emergence and widespread distribution of microbial resistance to antibiotics is world-wide challenge in the medical science. Consequently, the discovery and characterization of novel methods as new antibacterial agent for the prevention, and treatment of infection bacterial are urgently required .In this regard, Dermatocarpon miniatum is one the most species lichens that have been used in folk medicines and could be replaced by traditional antibiotics. Despite its reported benefits, there are reports associated with D. miniatum adverse effects. The above problems can be solved by encapsulating D. miniatum into nanoformulations. Integrating D. miniatum into nanocarriers may decrease its toxicity and improve the bioavailability, solubility and the antibacterial activity.Methods : In this study, the ZnO/GQDs nanocomposites (NCs) and D. miniatum grafted ZnO/GQDs NCs have been prepared via green method. The prepared products are characterized with XRD analysis, SEM and FTIR.The MICs of D. miniatum, D. miniatum/ZnO/GQDs -NPs were determined using the broth microdilution method as described by the CLSI standards.Results : The result of in-vitro experiments demonstrated that Interestingly, the linking of D. miniatum into ZnO/GQDs NCs demonstrated strong antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) compared to free D. miniatum. The MIC value of D. miniatum /ZnO/GQDs against MRSA was ۱۲۵ µg/mL, whereas the MIC value against D. miniatum was ۶۲۵ µg/mL.Conclusion : Finally, results this study reported that the D. miniatum /ZnO/GQDs–NCs formulation could be employed as a novel agent antibacterial to inhibit bacterial growth .

کلیدواژه ها:

antibacterial ، nanocarriers ، Methicillin-resistant Staphylococcus aureus ، Dermatocarpon miniatum