Study of Aqueous-Alcoholic Extracts of Peganum Harmala Plant on Growth Rate of Escherichia Coli Bacteria Isolated from the Stools of Children with Diarrhea

Background and Aim : Diarrhea is often induced by infection of digestive system .It is one of the major causes of death in children under ۶ years old in developing countries.Among the Bacterial of death in children under ۶ years old in developing countries .Among the Bacterial pathogens,E.coli is the most important causative agent of endemic and epidemic diarrhea around the word . Peganum harmala is a perennial plant with abundant green leaves that Belongs to the family Nitrariaceae.Due to the indiscriminate consumption of antibiotics and the increasing resistance of bacteria ,and to seek an appropriate substitute for antibiotics, we proceeded to investigate the effect of hydro alcoholic Peganum harmala extract on the growth rate of E.coli isolated from stool of children with diarrhea. Methods : This descriptive cross-sectional study was carried out on ۱۰۰ samples isolated from stools of children with diarrhea admitted. Following sample collection and culturing ,E.coli strains causing childrens diarrhea were identified.Results : All of the used antibiotics except ampicillin and tetracycline had a positive effect on E.coli . Of a total of ۱۰۰ samples collected ,۷۰ samples showed growth ,and of these ,۵۸ samples were related to Shigella.The results of this study showed that Peganum extract shows no effect on E.coli. Conclusion : The Peganum Harmala extract did not show any inhibitory effect on Escherichia coli bacteria. All antibiotics used on Escherichia coli bacteria were effective except for ampicillin and tetracycline that was resistant.

کلیدواژه ها:

Peganum Harmala ، PCR ، Extracts of Peganum