Determination of Host Range of a Specific Pseudomonas aeruginosa Bacteriophage

Background and Aim : Bacteriophages are advanced tools to combat the antimicrobial resistance of human opportunistic strains of Pseudomonas aeruginosa. Phages or bacteriophages have a limited hosting range that accurately identifies which bacteria to target. In this study, the host range of a specific Pseudomonas aeruginosa Bacteriophage on clinical samples of Klebsiella and Acinetobacter has been investigated.Methods : After isolation of specific phages of Pseudomonas aeruginosa from wastewater, purification was done. Then, the phage hosting range was investigated on two bacteria, Klebsiella and Acinetobacter. The plaque formed on the plate was evaluated using point method. First we cultivated bacterial strains in the TSB culture, then added ۱۰ microliters of bacteria in tubes containing ۵% Agar Agar Broth. In the next stage, a special phage of Pseudomonas aeruginosa was added in a point form. The plates were incubated for ۲۴ hours at ۳۷ ° C, plaque formation or non-plaque formation was investigated.Results : Pseudomonas aeruginosa hosting renge results showed that this phage does not have a very wide range of influence and did not have a clear plaque on the Klebsiella and Acinetobacter bacteria. With this experiment, we identified the proprietary phage against Pseudomonas aeruginosa strains to treat infections of this bacterium.Conclusion : The results showed that the isolated phage was highly specific and could be used as a smart weapon to replace most of the antibiotics resistant to this gram-negative bacterium to prevent Pseudomonas aeruginosa infections.

کلیدواژه ها:

Host range ، Bacteriophage ، Pseudomonas aeruginosa