A case control study of gut dominant bacterial phylum in people with type-۲ diabetes mellitus and nonalcoholic fatty liver disease

Background and Aim : Non-alcoholic fatty liver disease (NAFLD) is the main reason for chronic liver disease, with an incidence that is growing in parallel with the global rise in obesity and type ۲ diabetes mellitus (T۲DM). The role of the gut microbiome (GM) is gaining interest as a significant factor in NAFLD and T۲DM pathogenesis. This study aimed to contrasts the differences of dominant GM phyla among patients with NAFLD as compared to T۲DM and control groups.Methods : Group-specific primers were designed to target the ۱۶SrRNA genes of major bacterial phylum present in the gut. After the validation of their specificity, these primers were used in the real-time PCR quantification of phyla in the fecal samples of NAFLD patients with T۲DM, NAFLD patients without T۲DM, patients only with T۲DM, and healthy control subjects.Results : Bacteroidetes and Firmicutes phyla were significantly low in NAFLD patients with T۲DM (Firmicutes, ۲.۵۵ ± ۲.۲۵, Pv ۰/۰۰۰۲ and Bacteroidetes, ۱.۵۵ ± ۲.۲۹, Pv ۰/۰۰۰۷). In comparing bacteria phyla between NAFLD and NAFLD with T۲DM groups, there were significant differences in Firmicutes and Bacteroidetes (Pv <۰.۰۵). Furthermore, in comparison to bacteria phyla between T۲DM and NAFLD with T۲DM groups, there were significant differences in Firmicutes and Bacteroidetes (Pv <۰.۰۵).Conclusion : To conclude, our findings showed that the NAFLD patients with T۲DM were characterized by different gut compositions. This study supports the role of GM in the pathogenesis of T۲DM and NAFLD.

کلیدواژه ها:

Gut microbiota ، Non-alcoholic fatty liver disease ، Type ۲ diabetes mellitus