Comparison of bacterial diversity in the oral microbiota of multiple sclerosis patients and healthy people by the Denaturing gradient gel electrophoresis (DGGE) method.

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 172

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شناسه ملی سند علمی:

MEDISM22_152

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله:

Background and Aim : Microorganisms in oral cavity are called oral microbiota, since most bacterial genera are uncultivable, cultural independent methods based on ۱۶SrRNA due to identification of more genera. DGGE is one of these methods that is used for examination of bacterial population variety. Microbiota influence development systemic diseases such as pancreatic cancer, atherosclerosis, diabetes, parkinson, autism, alzheimer's, rheumatoid arthritis and multiple sclerosis (MS) through inflammation, immunomodulatory, metabolism etc. MS is an autoimmune disease and associated with chronic inflammation and demyelination. Bacterial infection is one of environmental factors of MS via their effects on inflammatory process include increase Th۱۷ and inflammatory cytokines. This study carried out for Comparison of oral microbiota in multiple sclerosis patients and healthy people by the DGGE method. Methods : Sampling include ۳۰patients and ۳۰ healthy controls was performed in MS patients center of Kermanshah University of Medical Sciences during october to march ۲۰۱۹. DNA was extracted from ۱ ml of saliva, electrophoresis of PCR products were performed on poly acrylamide gel. The desired bands were cut and sequencing was carried out.Results : DGGE images showed that this method can identify a greater number of bacteria genera or species compared with cultural methods. Staphylococcus, Bacteroides, Porphyromonas, Prevotella and Actinomyces genera were higher in the patients group and Micrococcus, Enterococcus and Lactobacillus genera higher in the healthy group, significantly. There was no significant difference among Bifidobacterium, Peptostreptococcus and Streptococcus. Sequencing results showed ۲۸ species were uncultivable and ۷ species with Accession number MW۸۸۰۹۱۹-۲۵ were submitted in https://www.ncbi.nlm.nih.gov/.Conclusion : Most bacteria that have been identified as inflammatory factors in other research were found to be more prevalent in the patient group in this study, indicating that they have a role in the inflammatory condition of MS patients. Furthermore, Lactobacillus, a beneficial bacterium, was shown to be less abundant in patients. Finally, the progression of the disease can be slowed by controlling oral infections and using helpful microorganisms with anti-inflammatory properties.

نویسندگان

Zahra Zangeneh

Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.

Ahya Abdi-Ali

Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.

Amirhoushang Alvandi

Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Ramin Abiri

Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.