Evaluation of colistin antibiotic resistance gene in k. pneumoniae

Background and Aim : increase antibiotic resistance in multidrug- resistant gram negative bacteria (MDR- GNB), presents significant health problems worldwide, since the vital available and effective antibiotics, including; penicillins, fluoroquinolones, aminoglycosides, and β-lactams; often fail to fight MDR Gram-negative pathogens as well as the absence of new antibiotics that can defeat these ‘superbugs’. All of these has prompted the reconsideration of old drugs such as polymyxins which have potential clinical toxicity, especially hepatotoxicity. However, although the polymyxins, especially colistin, are used as the last line of antimicrobial defense, the resistance against polymyxin in pathogensMethods : using disk diffusion method, we tried to Determining the prevalence of various types of resistance in Klebsiella pneumoniae, which causes many nosocomial infections, Use of the Phoenix device to determine the minimum inhibitory concentration for ۱۶ antibiotics and also using PCR method to try to identify its molecular mechanisms in different strains of bacteria isolated from several hospitals in Isfahan, including chromosomal coded resistance, mutated chromosomal mechanisms and transposon genes involved in the development of antibiotic resistance such as the MCR gene group, the synergistic effect of the compounds Antibiotics and non-antibiotics to the isolated strains of colistin resistant Results : All ۶۸ strains of the bacterium were isolated mdr, of which ۱۶ were colistin-resistant bacteria, indicating that the prevalence of colistin resistance among these bacterial strains was increasing, causing high mortality among hospitalized patients.Conclusion : All K. pneumoniae showed a multidrug-resistant phenotype, particularly to colistin. Colistin resistance was mainly associated with deleterious mutations and transposon in the mgrB gene and it is necessary to prevent the spread of these genes as much as possible by using new strategies such as the use of probiotics, etc.

کلیدواژه ها:

Gram-negative bacteria ، antibiotic resistance ، colistin ، MCR