Nano antimicrobial photodynamic therapy: An in vitro therapeutic application in plasma containing SARS-CoV-۲

Background and Aim : The ability of antimicrobial photodynamic therapy (aPDT) as a treatment approach and adjuvant therapy using Curcumin-Poly (Lactic-co-Glycolic Acid) nanoparticles (Cur@PLGA-NPs) was investigated to inactivate Coronavirus disease ۲۰۱۹ (COVID-۱۹) in plasma. Methods : Following synthesis and characterization of Cur@PLGA-NPs, the treated plasma samples with Cur@PLGA-NPs plus blue laser were exposed to Vero cells. Eventually, cell cytotoxicity and apoptotic effects of treated Vero cells were evaluated. Results : Different concentrations of Cur@PLGA-NPs (۳, ۵, ۷, and ۱۰% wt.), the irradiation times of blue laser (۱, ۳, and ۵ min), and aPDT with the maximum dosed of blue laser light (۵۲۲.۸ J/cm۲) plus ۱۰% wt. Cur@PLGA-NPs had no cytotoxicity. Although there were significant cell degradation and apoptotic effects in treated Vero cells with treated plasma using ۱۰% wt. Cur@PLGA-NPs, and a blue laser at an energy density of ۵۲۲.۸ J/cm۲, no visible changes in cells and apoptosis were observed following aPDT. Conclusion : aPDT exhibited in vitro anti-COVID-۱۹ activities in the treated plasma containing SARS-CoV-۲ without Vero cell apoptosis and any adverse effects on plasma quality in aPDT-exposed plasma.

کلیدواژه ها:

Antimicrobial photodynamic therapy ، Coronavirus ، COVID-۱۹ ، Curcumin ، PLGA ، SARS-CoV-۲