Investigation the effects of Gold nanoparticles in combination with hyperthermia and radiotherapy on CT-۲۶ cell line

Background & Aims: Colorectal cancer (CRC) is the fourth most widely diagnosed cancer worldwide and manifests as a malignant neoplasm in the mucosa of the colon or the rectum. Nanoscale delivery systems, or nanotherapies, are increasing in importance as vehicles for antineoplastic agents because of their potential for targeting and multifunctionality. The aim of this paper was examining the combined impacts of Gold nanoparticles (Au NPs), hyperthermia (H), and irradiation (R) on an increment of CT-۲۶ cells.Methods: The MTT assay was employed to assess the antiproliferative effects of Gold NPs (۲۰, ۴۰, and ۸۰ μg/ml), hyperthermia (۴۱ C for ۱ h), and irradiation (۲۰۰ cGy). Moreover, the perniciousness was estimated through the survival capability of cells, and apoptosis, ROS production.Results: A significant decrease in proliferation index, a significant increase in apoptosis of CT-۲۶ cells, a significant increase in ROS formation were recorded in the adjacency of CT-۲۶ cells by a combination of Gold NPs (۸۰ mg/ml) and R + H compared to control and other treatments.Conclusion: Based on these observations, a combination of Gold NPs, hyperthermia, and irradiation could suppress the growth of CT-۲۶ cells.

کلیدواژه ها:

Nanotherapy ، Hyperthermia ، Radiotherapy ، CT۲۶ cell line