Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress

سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 150

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJBMS-21-12_007

تاریخ نمایه سازی: 27 شهریور 1400

چکیده مقاله:

Objective(s): Obstructive sleep apnea (OSA) is confirmed to cause lesions in multiple organs, especially in the lung tissue. Tempol is an antioxidant that has been reported to restrain inflammation and oxidative stress, with its role in OSA-induced lung injury being unclear. This study aimed to investigate the beneficial effect of tempol on chronic intermittent hypoxia (IH)-induced lung injury.Materials and Methods: A rat model of OSA was established by IH. There were four groups: normal air (NA), IH, IH+tempol, NA+tempol. Inflammatory response was evaluated by TNF-α, IL-۱β, and IL-۶ levels. Oxidative stress was detected by MDA and GSH levels, and SOD activity. The protein levels were assessed by Western blot. DNA binding activity of NF-κB or Nrf۲ was determined by electrophoretic mobility shift assay.Results: According to the results, tempol administration alleviated pathological changes of the lung tissue, decreased leukocyte count and protein content (P<۰.۰۰۱) in bronchoalveolar lavage fluid (BALF). Inflammation response in lung tissue induced by IH was suppressed by tempol as evidenced by decreased levels of TNF-α, IL-۱β, and IL-۶ (P<۰.۰۰۱) and protein levels of COX-۲ and iNOS (P<۰.۰۰۱). Moreover, tempol inhibited oxidative stress in lung tissue by down-regulating the MDA level (P<۰.۰۰۱) and enhancing SOD activity (P<۰.۰۰۱) and the GSH level (P<۰.۰۵). In addition, tempol repressed inflammation response via inactivation of the NF-κB pathway. Furthermore, the results suggested that tempol repressed oxidative stress by activating the Nrf۲/HO-۱ pathway. Conclusion: Our findings suggest that tempol effectively relieves OSA-induced lung injury.

نویسندگان

Yeying Wang

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Bing Hai

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Li Ai

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Yu Cao

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Ran Li

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Hui Li

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

Yongxia Li

Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan ۶۵۰۱۰۱, People’s Republic of China

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Punjabi NM. The epidemiology of adult obstructive sleep apnea. Proc ...
  • Lu W, Kang J, Hu K, Tang S, Zhou X, ...
  • Jun JC, Chopra S, Schwartz AR. Sleep apnoea. Eur Respir ...
  • Dylag AM, Mayer CA, Raffay TM, Martin RJ, Jafri A, ...
  • Zhang J, Zheng L, Cao J, Chen B, Jin D. ...
  • Skelly JR, Edge D, Shortt CM, Jones JF, Bradford A, ...
  • Troncoso Brindeiro CM, da Silva AQ, Allahdadi KJ, Youngblood V, ...
  • Guo H, Cao J, Li J, Yang X, Jiang J, ...
  • Ramond A, Godin-Ribuot D, Ribuot C, Totoson P, Koritchneva I, ...
  • Liu Y, Li Y, Li N, Teng W, Wang M, ...
  • Wang TY, Zhou QL, Li M, Shang YX. Shikonin alleviates ...
  • Zhao H, Zhao M, Wang Y, Li F, Zhang Z. ...
  • El-Sayed NS, Mahran LG, Khattab MM. Tempol, a membrane-permeable radical ...
  • Teke Z, Kabay B, Ozden A, Yenisey C, Bir F, ...
  • Cuzzocrea S, McDonald MC, Mazzon E, Filipe HM, Centorrino T, ...
  • Wang Y, Chai Y, He X, Ai L, Sun X, ...
  • Zhao C, Sun J, Fang C, Tang F. ۱,۸-cineol attenuates ...
  • Minoguchi K, Tazaki T, Yokoe T, Minoguchi H, Watanabe Y, ...
  • Yokoe T, Minoguchi K, Matsuo H, Oda N, Minoguchi H, ...
  • Mehra R, Storfer-Isser A, Kirchner HL, Johnson N, Jenny N, ...
  • Copray JC, Mantingh I, Brouwer N, Biber K, Kust BM, ...
  • Kleinert H, Pautz A, Linker K, Schwarz PM. Regulation of ...
  • Guzik TJ, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide ...
  • Chen Q, Wang N, Zhu M, Lu J, Zhong H, ...
  • Lu W, Kang J, Hu K, Tang S, Zhou X, ...
  • Chen TI, Chen MY. Zinc Is Indispensable in Exercise-Induced Cardioprotection ...
  • Zhao YN, Wang HY, Li JM, Chen BY, Xia G, ...
  • Hou YJ, Zhao YY, Xiong B, Cui XS, Kim NH, ...
  • Valko M, Jomova K, Rhodes CJ, Kuca K, Musilek K. ...
  • Rhee JW, Lee KW, Kim D, Lee Y, Jeon OH, ...
  • Wang SS, Purdue MP, Cerhan JR, Zheng T, Menashe I, ...
  • Yan F, Chen Y, Azat R, Zheng X. Mulberry Anthocyanin ...
  • Lee MS, Lee B, Park KE, Utsuki T, Shin T, ...
  • Li CG, Ni CL, Yang M, Tang YZ, Li Z, ...
  • نمایش کامل مراجع