A study of the transformation of umbilical cord mesenchymal stem cells by interferon-gamma

Objective(s): Mesenchymal stem cells (MSCs) exist in almost all tissues. Their unique nature is completed by their immunomodulatory functions, holding promise for the treatment of many diseases. An inflammatory environment precedes the immunosuppressive abilities of MSCs and this study was intended to better understand how umbilical cord MSCs (UCMSCs) react to the process of inflammation, regarding their basic characteristics and behavior when primed with the key pro-inflammatory cytokine, Interferon-γ (IFNγ).Materials and Methods: Human MSCs from the umbilical cord were isolated, expanded, and treated with IFNγ. Primed cells were analyzed to define their ability to form colonies, their morphology, differentiation potential, proliferation, and apoptosis rate.  Results: UCMSCs treated with IFNγ changed their fibroblast-like morphology and retained the expression of typical MSCs markers. IFNγ treated UCMSCs had significantly higher MFI levels regarding the expression of HLA-I (۹۸۰.۴۳ ± ۵۵۶.۶۴) and PD-L۱ (۵۹۸.۰۴ ± ۴۱۶.۹۰) compared with the control cells (۱۴۴.۹۷ ± ۷۸.۵ and ۱۲۲.۰۵ ± ۱۰۳.۸۳, respectively; p <۰.۰۱). Under the influence of IFNγ, the cells had a lower population doubling time compared with the control cultures (۵۰.۳۴۵ ± ۹.۱۵۵ versus ۶۱.۱۳۵ ± ۲۱.۱۱۰, respectively; p <۰.۰۱) and higher numbers of colony-forming unit-fibroblasts (۲۶.۰ ± ۱۲.۲ versus ۱۰.۲ ± ۸.۰, respectively; p <۰.۰۵). The primed MSCs could not undergo osteogenic and adipogenic differentiation. IFNγ increased the percentage of cells in the apoptotic state on day eight (۲۹.۴۷۰ ± ۶.۵۹ versus ۱۵.۷۰۸ ± ۶.۱۹۰, respectively; p <۰.۰۱).Conclusion: The properties of UCMSCs can be influenced by the pro-inflammatory cytokine IFNγ.

کلیدواژه ها:

Cytokine ، Differentiation ، Inflammation ، PD-L۱ ، Proliferation ، Stem cells