Production of Lipase by Isolated Halophile, Halobacillus sp. Strain AR۱۱ from International Miankaleh Wetland
محل انتشار: مجله منابع ژنتیک، دوره: 7، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 232
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شناسه ملی سند علمی:
JR_SGR-7-2_004
تاریخ نمایه سازی: 10 شهریور 1400
چکیده مقاله:
Lipases are particularly important because they specifically hydrolyze acyl glycerol, oils, which are of great interest for different industrial applications. Some halophilic microorganisms produced some lipases. Halophilic bacteria have greater ability to produce salt and thermo tolerant enzymes like amylases, proteases and lipases. Most industrial enzymatic functions may be stopped by concentrated salt solutions and high temperatures; therefore, halophilic enzymes that have optimal activity at a vast range of temperature, pH and ionic strength, would be considered as suitable biocatalysts in industrial processes. The aim of this study was to isolate and study the halophilic lipase producing bacteria from the Miankaleh Wetland. The lipase activity was measured using titrimetric methods. Three halophilic strains (AR۱۱, AR۱۸ and AR۲۸) were isolated from the Miankaleh wetland and were screened for the production of hydrolytic enzymes and lipolytic activity. Among three isolates, one strain was selected for identification using the molecular methods and some morphological characteristics. The bacterium Halobacillus truperi AR۱۱ with ۱.۸۲ ± ۰.۱ U/mL lipase activity was selected as the highest lipase producing isolate. Lipase enzyme produced by this potential isolate was also characterized for determining its optimal activity. Effect of different pH, NaCl concentration and temperature on lipase activity was determined. The optimum pH for AR۱۱ was found to be ۹, while the optimum temperature and NaCl concentration for lipase activity was found to be ۴۵ºC and ۵ % NaCl, respectively. This is the first report of isolation and molecular identification of lipase producing halophilic bacteria from the Miankaleh wetland.
کلیدواژه ها:
نویسندگان
Sedigheh Rajaei-Maleki
Department of Microbiology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran
Bagher Seyedalipour
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Salman Ahmady-Asbchin
Department of Microbiology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran
Gholamhossein Riazi
Biochemistry Department, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
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