Protein pattern of bread wheat cultivars before and after flowering using SDS-PAGE technique

Protein patterns have been widely used to determine genetic variation in cereals, especially wheat. This study was carried out at Payame-Noor Asadabad University in ۲۰۱۴. To study genetic diversity and protein pattern in bread wheat cultivars before and after flowering ۱۳ bread wheat cultivars were used to prepare protein patterns based on SDS-PAGE electrophoresis. The highest genetic distance before flowering was between Zare and Gascogen (۸ units). The lowest genetic distance before flowering was between cultivars with a distance equal to zero. This result indicates high genetic similarity between wheat cultivars. The most similarity between the cultivars is the value of one, such as Pishgam and Sayson (one means one hundred percent similarity) or Pishgam and Bezustaya (one). The least similarity is ۰.۲ between Zare and Gascogen. Based on cluster analysis, ۱۳ bread wheat cultivars were divided into three groups. The results after flowering were largely similar to the protein pattern before flowering. The most similarity between the cultivars is the value of one. Between Pishgam and Sayson is one (one hundred percent similarity) or Pishgam and Bezustaya (one). The lowest similarity was observed between Zare and Gascogen cultivars with zero and ۰.۲۵ between Zare and other cultivars. Based on cluster analysis, ۱۳ wheat cultivars were grouped into four clusters. Increasing one group indicates the effect of some proteins that are effective in the developmental stages before and after wheat flowering.

کلیدواژه ها:

Bread wheat ، genetic diversity ، biochemical markers ، clustering