Stimulation of secondary metabolites production in Zataria multiflora cell suspension culture through elicitation with salicylic acid

Zataria multiflora (Lamiaceae) is medicinal plant with a wide range of biological properties due to having phenolic compounds such as polyphenols and terpenoid compounds. Induction of secondary metabolites in plant cell culture systems by elicitors is one of the most important strategies to improve the production of medicinal metabolites. In the present study a cell line was established from Z. multiflora in B۵ medium and the effects of salicylic acid (SA) at ۹۰, ۱۸۰ and ۳۶۰ μM on suspension-cultured cells was evaluated. The cells were treated at their logarithmic growth phase for ۳ days and the activity of regulatory and antioxidant enzymes, also the major phenolics were determined by HPLC and spectroscopy techniques. The results showed at ۹۰ and ۱۸۰ μM of SA, growth of Zataria cells significantly reduced (۸۷.۶۲% and ۷۲.۶۰% of the control group, respectively). Salicylic acid treatment lead to increase in the activities antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) as well as nitric oxide (NO) content compared with control. Increase of signal molecules in SA elicitation is important, because these molecules trigger signal transduction pathways to induce regulatory enzymes such as PAL and TAL to increase the secondary metabolites accumulation in Zatria cells. SA at ۹۰ and ۱۸۰ μM concentrations significantly enhanced the activity of PAL (about ۱.۲ fold), TAL (۱.۳ - ۱.۴ fold) and PPO (۱.۵ - ۱.۷ fold) as well as total contents of phenol (۵.۷۹ and ۴.۳۸ mg g-۱ FW) compared to control respectively. PAL and TAL enzymes involved in the phenolic pathway which lead to shift from primary to secondary metabolism. SA induced the production of ۴-hydroxy benzoic acid, benzoic acid, epicatechin and syringic acid in Zataria cell. This study indicates that cell suspension culture could be used as an efficient system for sustainable production of medicinal compounds.

کلیدواژه ها:

Zataria multiflora ، ، abiotic elicitor ، nitric oxide ، phenolic compounds ، antioxidant enzymes