Evaluation of cyanobacterial treatments on the antioxidant enzymes activities in Melissa officinalis L. as a medicinal plant

The use of microalgae and their active ingredients is important in order to increase the essential oil of some medicinal plants. Melissa officinalis L. is a perennial plant which its leaves and aerial parts have medicinal value. The aim of this study was to evaluate the changes in the activity of antioxidant enzymes (peroxidase and superoxide dismutase) and the amount of H۲O۲ in the medicinal plant under the influence of cyanobacteria (Nostoc sp. and Spirulina sp.) as elicitors after ۸۳ days. Based on the results, Nostoc-treated plants showed the lowest activity of peroxidase and superoxide dismutase enzymes and the highest amount of H۲O۲. Also, in plants treated with Spirulina, the amount of H۲O۲ increased because of reducing the activity of antioxidant enzymes. The highest activity of antioxidant enzymes and the lowest amount of H۲O۲ were obtained in control plants (water- treated plant). Therefore, treatment of Melissa officinalis with Nostoc and Spirulina cyanobacteria has led to stress conditions (increased H۲O۲) in this plant. Considering that one of the ways to increase the essential oil in medicinal plants is to create stress conditions in them, so it seems that the active component has been increased in plants treated with these cyanobacteria, which requires further analysis in this field.

کلیدواژه ها:

Nostoc ، Spirulina ، Peroxidase ، Superoxide desmutase ، Melissa officinalis