Comparison of Cultivation of Plant Tissues of Healthy and gall branches in the Salix babylonica

The lateral and apical buds of the weeping willow tree change their growth against the attack of the eriophid mide, creating a broom-like gall that the parasitic insect uses to feed or as a shelter. In order to understand the changes in the gall, healthy branches of the weeping willow tree were removed by removing the plant tissue mite. Detergents are separated from the branches and after disinfection with ۲۰% sodium hypochlorite and ۷۰% ethanol, washed with distilled water and transferred to MS culture medium under Laminar hood and in optical conditions, ۸ hours of darkness and ۱۶ hours of light were maintained at ۲۵° C for one month. Cultivation results showed that the cultured tissue from the gall branches grew in the same way as the plant, indicating that the gall agent, by induction between specific genes, altered the process of expression of specific genes, which even With its physical elimination, its internal changes have been institutionalized. The node from the gall branches grew in the same way in the culture medium and were accompanied by deformation of the leaves and reduction of the distance between the node and the size of the leaves, and these branches, unlike the branches from the nodes, Healthy, they had no roots. Therefore, the changes caused by the gall insect are internal, and its physical removal does not change the process of gall formation. On the other hand, due to the lack of root formation in the culture medium, it is shown that the amount of auxin hormone, which is the root cause factor in these gall branches, has decreased.

کلیدواژه ها:

Eriophyte mite ، MS culture medium ، Rooting ، Tissue culture ، Willow