Biochemical study of secretory compounds from germinating seeds of Clover and Fenugreek under cold stress

During germination, seeds of papilionaceae family secrete certain compounds to symbiosis with specific bacteria to stabilize nitrogen. In this study, the effect of cold treatment (۱۰±۲C˚) on the biochemical compounds secreted from the germinating seeds of Clover and Fenugreek compared to normal temperature (۲۵±۳ C˚ ). After fourteen hours, the seeds secretions were dried at room temperature then mixed with potassium bromide in a ratio of ۱:۱۰۰. The resulting tablets were studied by FTIR and their graphs were drawn using Excel software. Examination of the obtained peaks from the secreted compounds showed that Clover seeds were most affected than Fenugreek. The change in position of the FTIR peaks in the secreted samples showed the presence of different compounds. The strong bands at ۳۲۰۰- ۳۵۰۰ cm−۱ indicated the presence of phenol and alcohols with free OH group. The vibrational peaks at ۲۸۰۰- ۳۰۰۰ cm−۱ showed the presence of alkane group in lipid compounds. The bands at ۱۵۰۰- ۱۷۰۰ cm−۱ reveal the protein compounds including COO – group. The absorption peaks at ۶۰۰- ۸۰۰ cm−۱ correspond to the =CH group of terpene compounds. Cold treatment increased the concentration of these compounds in the secreted composition of the both seeds, but this increase was more in Clover than Fenugreek. Additional studies are necessary to investigate the effect of cold treatment on the relationship between symbiosis of the roots of these plants with nitrogen-fixing bacteria.

کلیدواژه ها:

Papilionaceae family ، Symbiosis ، Concentration ، Phenol