Effect of magnetic field and light spectrums on the growth and antioxidant capacity of Hyoscyamous reticulatus callus tissue

Hyoscyamus reticulatus L. is a medicinal plant belonging to Solanaceae family and has analgesic, antispasmodic and antichlorogenic capacities. Magnetic field (MF) is an abiotic stress which is used as a technique in biological studies such as modification of antioxidant capacity and medicinal metabolites. Light spectrums are as a suitable light source for plant growth and can induce plant growth and development. In this study, sterilized seeds were put in Murashige and Skoog (MS) medium. For callus induction, hypocotyl explants were placed in MS culture medium containing ۲, ۴-D = ۰.۵ mg L-۱ and BA = ۰.۵ mg L-۱ hormones. After ۳ weeks, the calli were subcultured under different MF intensities (۰ and ۴ militesla (mT). Then, calli were transferred to the growth chamber and subjected to different light spectrums in four treatments (control, MF, red-blue (RB), MF + RB). The results showed that RB light induced fresh weight of callus tissue (۱۸.۳%), and the highest fresh weight (۶۰.۱۱%) was obtained under RB+MF spectrum. MF treatment caused a significant increase in growth index which induced an ۷۵.۸۸% raise comparing to the control. Free radical scavenging activity (DPPH) increased under the influence of MF and RB spectrums, and the highest DPPH activity (۸۴.۷%) was observed in the MF treatment with an ۳۱.۸% increase compared to control. It seems that the MF with the regulation of antioxidant defense system can induce the growth of callus tissue.

کلیدواژه ها:

Red-blue light spectrum ، Fresh weight ، Radical scavenging activity