Effect of BAP and ۲-۴-D hormones on the amount of phenolic and flavonoid Compounds of Hypericum perforatum calli in vitro conditions

Plants are able to produce some valuable bioactive compounds, such as phenolic compound in vitro conditions .Polyphenols and phenolic compounds are plant secondary metabolites, which have antioxidant activity. Hypericum perforatum (Hypericum; Hypericaceae) an important medicinal plant and are used in the pharmaceutical industry. Extracts and compounds from plants belonging to this family have important properties such as antidepressant, antibacterial, anti-cancer and anti-inflammatory. In ۲۰۲۰, to evaluate the effects of benzyl amino purine (BAP) and ۲,۴-Dichloro phenoxy acetic acid (۲-۴-D) Hormones on values of phenol and flavonoids of calli from the leaf explants of H. perforatum, an experiment was performed at the University of Zabol a factorial in a completely randomized design with three replication. In the present study, the amount of phenolic and flavonoid compounds in calli extracts obtained on MS medium containing different concentrations of ۲-۴-D (۰.۵ and ۱ mg/L-۱) and BAP (۰.۵ and ۱ mg/L-۱) hormones were determined by Folin-Ciocaltiu and Aluminum Chloride colorimetric methods, respectively. The results of mean comparison showed that the highest amount of flavonoids (۱۱.۲۷ mg/gDW) and phenol (۲۲.۱۵ mg/-gDW) was obtained from MS medium containing ۱ mg/l-۱ BAP+۰.۵ mg/l-۱ ۲.۴-D. Consequently, these results can provide that by optimizing the concentrations of Elicitors such as BAP and ۲-۴-D hormones, on the calli of the medicinal plant H. perforatum, the production of secondary metabolites of this plant can be increased in vitro.

کلیدواژه ها:

Optimization ، Hormone treatments ، Tissue Culture ، Leaf explant