Inhibitory effect of Hyoscyamus senecionis extract on alpha-amylase enzyme

Diabetes is a chronic disease that occurs when the pancreas does not produce enough insulin or the body cannot use the insulin it produces effectively. One of the most important issues in the treatment and control of type ۲ diabetes is the reduction of hyperglycemia after consuming sugars, which is possible by inhibiting enzymes such as alpha-amylase and alpha-glucosidase. The use of plants has been common in the past for many disorders and diseases. Today, due to the side effects of chemical drugs and the high cost of these drugs, the use of herbal medicines in medicine and traditional medicine has increased significantly compared to the past. The Solanaceae is one of the most important latent genus in terms of medicine and nutrition because it contains important alkaloids such as hyoscyamine, atropine and scopolamine. Hyoscamus senecionis belongs to the genus Solanaceae. This family is mainly distributed in the tropics and the main origin of this plant is the United States. The aim of this study was to investigate the anti-diabetic effects of methanolic extract of H. senecionis leaf from Solanaceae genus. This experimental study investigates the anti-diabetic properties by measuring the inhibition of α-amylase. Alpha-amylase inhibition test was performed by measuring the reduction of oligosaccharide release power from starch solution and to evaluate the effect of methanolic extract of H. senecionis leaf on alpha-amylase inhibition process, the fluorescence intensity of the samples was measured. In this experiment, the concentration of the extract required to inhibit ۵۰% of the enzyme activity (IC۵۰) was obtained and compared with the required amount of acarbose as a positive control. Leaf methanolic extract with IC۵۰ = ۸۱۱.۶۲ μg / ml has anti-diabetic properties compared to acarbose with IC۵۰ = ۲.۶۸ μg / ml.

کلیدواژه ها:

Diabetes ، Alpha Glucosidase ، Solanaceae