Effect of cytotoxicity of silver nanoparticles synthesized with Thymus vulgaris extract on Nalm۶ leukemia cell line

Thymus vulgaris is a perennial plant with high antioxidant properties that grows in the Mediterranean regions. Today, with the help of nanotechnology, biocompatible metal nanoparticles can be synthesized from metals such as gold, silver and copper using compounds in plant extracts. Synthesis of silver nanoparticles by green method has various advantages that make these nanoparticles useful for interdisciplinary studies.The Nalm۶ cell line belongs to acute lymphoid leukemia that are used for in vitro studies such as apoptosis induction in leukemia cells. In this study, we first synthesized silver nanoparticles with Thymus vulgaris extract by green synthesis method. Then, the nanoparticles were tested and approved by D.L.S technology. The synthesized nanoparticles along with the extract of Thymus vulgaris plant were exposed to Nalm۶ cells at various hours and the amount of cytotoxicity of MTT test was examined. The obtained information indicates the successful synthesis of silver nanoparticles with the extract of Thymus vulgaris plant. The size of the synthesized nanoparticles is between ۲۵ and ۴۵ nanometers. The IC۵۰ value for Nalm۶ cells also decreased with increasing time. Thymus vulgaris extract and nanoparticles also induced necrotic apoptosis in Nalm۶ cells. This study confirms the effect of nanoparticles and Thymus vulgaris plant on the induction of cell death in the studied cells and introduces a potential factor for the treatment of these diseases.

کلیدواژه ها:

Thymus vulgaris ، Nalm۶ ، Green synthesis