Isolation of carotenoid pigments from Staphylococcus aureus isolated from marine sponge

Staphyloxanthine is a Staphylococcus aureus membrane-bound carotenoid with potential of antioxidant and anti-cancer. The genome sequence was obtained from the NCBI Genebank database and analyzed by antiSMASH software. S. aureus isolated from marine sponges were cultured in ۱۰۰ cc of tryptose soy broth and incubated at ۳۷ ° C for ۲۴ hours. The bacterium was characterized by biochemical tests such as mannitol salt agar, catalase and oxidase, centrifuged, the precipitate was suspended in ۱۵ cc of methanol and incubated at ۵۵ ° C for ۳۰ min, the suspension was centrifuged at ۸۰۰۰ rpm for ۱۰ minutes.The supernatant was purified using a thin layer chromatographic and silica gel column (Merck ۶۰ GF۲۵۴) chromatography (۱۳ cm height, a ۱ cm width) by ethyl acetate and ethanol solvents (۹:۱). Evolution of the genome by antiSMASH software revealed that the genome of this bacterium contain genes encoding PKS type III enzymes, NRPS, terpene, siderophore and class I lentippeptide. The carotenoids shwed photo absorption in ۴۶۰, ۴۶۲, ۴۳۷, ۴۵۰, ۳۷۳ and ۲۷۵ nm. The yellow carotenoid band was observed in thin layer chromatography. Advance surveying is under progress.

کلیدواژه ها:

Staphyoxanthin ، anticancer ، antioxodant ، antibacterial