Prevalence of Vibrio parahaemolyticus species and frequency of tdh pathogenic gene in strains isolated from Persian Gulf fish and shrimp

Vibrio are gram-negative, curved bacilli, halotolerant bacteria that live in sea waters. The present study was performed to determine the prevalence of Vibrio parahaemolyticus species and the frequency of tdh pathogenic gene in Vibrio parahaemolyticus isolated from fresh fish and shrimp samples and salt.In this descriptive cross-sectional study, ۱۱۸ samples consisting of fresh and salted fish and shrimp were collected from different regions of the Persian Gulf and immediately transferred to the lab. They were enriched and ۱ g of the samples were poured into tubes containing alkaline peptone water and transferred for ۶ hours at ۳۷ ° C. After this incubation period, the samples were occulated on TCBS and incubated for ۲۴ hours at ۳۷ °C. Initial identification of Vibrio parahaemolyticus based on the observation of green colonies that are not capable of consuming sucrose sugar and were Gram negative curved bacteria. In Vibrio parahaemolyticus, tdh gene was also identified on isolates using PCR. The results of the present study indicate the high prevalence of Vibrio parahaemolyticus in samples of fresh shrimp and salted fish. The results of biochemical tests showed that ۳۶ out of ۱۱۸ samples (۳۰.۵%) were infected with Vibrio parahaemolyticus. Therefore, it is suggested to use PCR method as a safe, accurate and fast test for detecting Vibrio species in seafood, in order to control the health of seafood in terms of the presence of Vibrio species.

کلیدواژه ها:

Vibrio parahaemolyticus ، Seafood ، Shrimp and fish ، PCR ، Pathogenic bacteria