Comparing the cytotoxic effects of ellagic acid derivatives on HT-۲۹ Cells

Ellagic acid, a natural phenol with valuable pharmacological activities, is frequently found in fruits, vegetables and nuts. Urolithins are main ellagic acid metabolites that induce chemopreventive and anticancer effects in vitro and in vivo. Colon cancer is among the five most common malignancies in the world. Great efforts have been undertaken to introduce novel and more effective compounds against aggressive colon cancer cells. In the present study, we evaluated and compared the effects of urolithins A, B, and the methylated form of urolithin A (UA,UB, mUA, respectively) on human colon cancer cells. After UA, UB and mUA were synthesized, HT-۲۹ cells, a human colon cancer cell line, were treated with increasing concentrations of these three agents for four consecutive days. To note, ۰.۴% DMSO was used as control treatment. For viability assessment of cells, alamar blue was used and optical density of cells was detected at ۶۰۰ nm. Determination of cell viability ۹۶ h after administration of ۱۰ μM UA, UB and mUA indicated that ۶۹%, ۹۱%, and ۱۰۰% of cells were alive, respectively. Regarding ۲۰ μM concentration, viability of cells was calculated as ۷۲%, ۸۵% and ۹۸% for UA, UB and mUA, respectively. In addition, upon ۹۶ h treatment with ۴۰ μM of UA, UB and mUA, cell viability was as ۷۲%, ۶۰% and ۹۶%, respectively. The highest cytotoxic effects were observed ۴ days after treatment with ۸۰ μM UA, UB and mUA, as cell viability was dramatically decreased down to ۵۶%, ۵۰% and ۶۷%, respectively. To sum up, the current findings revealed that in concentrations < ۸۰ μM, UA induced more toxic effects in comparison with other ellagic acid derivatives. Although, more research is required to confirm our results on other colon cancer cell lines.

کلیدواژه ها:

ellagic acid ، urolithin ، colon cancer ، cytotoxicity