Study of association between Circular RNA۰۰۰۲۸۴, miR-۵۰۶ and SNAIL -۲ expression in breast cancer

Breast cancer is the most common malignancy among women and along with lung and colon cancer is one of the most common cancers in the world. Therefore, finding solutions such as early diagnosis of the disease or targeted treatment can reduce the death rate. Today, biomarkers have received considerable attention for the diagnosis, prognosis and treatment of breast cancer. One of this Biomarkers is non-coding RNAs. In this study, the expression of non-coding RNAs including miR-۵۰۶ and Circular RNA ۰۰۰۲۸۴ and their target gene SNAIL-۲ was examined, also the relationship between their expression in breast tumor tissue was assessed, to nominate a possible biomarker for prognosis or a therapeutic goal. Initially, total RNAs were extracted from ۳۰ breast tumor tissue samples and their adjacent normal tissue. Then relevant cDNAs were synthesized and finally, the expression of each was examined with the using of Real-time PCR technique. According to the studies, circular RNA۰۰۰۲۸۴ expression has increased in breast tumor tissue compared to the adjacent normal tissue and decreased expression of miR-۵۰۶ was seen, also the expression of the SNAIL-۲ gene in the tissues of patients has increased compared to the adjacent normal tissue. In this study, the expression of circular RNA۰۰۰۲۸۴, miR-۵۰۶, and SNAIL-۲ depicted that, circular RNA ۰۰۰۲۸۴ sponged the miR-۵۰۶ in the tumor tissue of patients with breast cancer. the target mRNA of miR-۵۰۶, which is SNALI-۲, has increased. the SNAIL-۲ is an EMT factor that leads to the epithelial to mesenchymal transition (EMT), consequently will cause to malignancy of tumor tissue in patients. Hence circular RNA۰۰۰۲۸۴ and miR-۵۰۶ can be nominated as prognostic biomarkers in breast cancer.

کلیدواژه ها:

Cancer ، Non-Coding RNA ، Biomarker