Investigating the Effect of Methotrexate on Expression Changes of LncRNA CASC۱۵ in Acute lymphoblastic leukemia (Jurkat E.۶.۱)

Acute lymphoblastic leukemia (ALL) is the most common cancer in children. Methotrexate is produced and used for chemotherapy, either alone or in combination with other chemotherapy drugs. It is effective for the treatment of some cancers, including breast, head and neck, leukemia, lymphoma, lung, osteosarcoma, and bladder cancer. CASC۱۵ is one of the important LncRNAs that expresses changes in most cancers. The aim of this study was to evaluate the effect of MTX drug on the expression of LncRNA CASC۱۵ in acute lymphoblastic leukemia in the Jurkat E۶.۱ cell line. first, MTX drug was prepared as a drug. Different doses of MTX were examined with the MTT test. Then, the MTX was provided at ۱ and ۱۰ doses as optimal concentrations. Then, the Jurkat E۶.۱ was cultured in two doses at ۷۲ hours with MTX. then RNA extraction and cDNA synthesis were down, and the expression of LncRNA CASC۱۵ and GAPDH gene was evaluated as housekeeping gene by Real Time PCR. Finally, the results of Real Time PCR were analyzed by Rest ۲۰۰۲ Software. The Results of the research showed that the expression of LncRNA CASC۱۵ significantly decreased after treatment with MTX at ۱ and ۱۰ doses in ۷۲ with (p-value < ۰.۰۰۱) compared to non-MTX samples .According to the results, it has been found that doses of ۱ and ۱۰ MTX in ۷۲ hours are the optimal doses and time of the effect of this drug. The expression of LncRNA CASC۱۵ at the indicated concentrations and time were ۰.۴۱۷ and ۰.۶۵۲. Therefore, considering the oncogenic role of CASC۱۵ in leukemia and reduced gene expression after drug treatment compared to the control group, it can be concluded that MTX can be an effective drug in controlling the expression and inhibition of oncogene genes in cancer.

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GAPDH ، housekeeping ، cDNA