Synthesis of Silver Nanoparticles Using Green Method of Plant Extract (Campsis radicans ) an their Antibacterial Effects

There are many different methods for production of silver nanoparticles ( Ag-NPs ). But using of plants in the synthesis of nanoparticles due to a cost effective and ecofriendly approach has been extensively noticed. In this research study, the synthesis of green silver nanoparticles using the extract of Campsis radicans and the study of antimicrobial properties as well as the study of secondary metabolites of this plant have been reported for the first time. After extraction and synthesis to evaluate the properties and confirm the synthesis of UV- noticeable spectroscopy (UV-Vis(, Fourier change infrared spectroscopy (FT-IR), X-beam diffraction (XRD), transmission electron microscopy (TEM). The antibacterial properties of nanoparticles synthesized on Staphylococcus aureus (ATCC۲۵۹۲۳) and Escherichia coli (ATCC۲۵۹۲۲) were also evaluated to measure MIC (minimum inhibitory concentration) and MBC (minimum lethal concentration). Finally, aluminum chloride (TFC) and fulline save cultive (TPC) were used to measure flavonoids and total phenols, respectively. FT-IR spectroscopy uncovered that AgNPs were functionalized with biomolecules that have essences amine gathered, carbonyl gathering, OH gatherings, and other settling useful gatherings. An assimilation band with a ۴۵۰ nm pivot was watching, which is identified with the Surface Plasmon Resonance (SPR) of the silver nanoparticles. The structure and synthesis of silver nanoparticles were dissected by XRD which indicated that the AgNPs are crystalline in nature and have face-focused cubic (FCC) geometry. The morphological investigation of silver nanoparticles utilizing TEM proposes that the nanoparticles are circular with a distance across of around ۷۵ nm. Our information demonstrated absolute phenol substance in aqueous extracts of flowers of Campsis radicans was ۵.۳۱ ± ۰.۱ mg GAE/g dry plant materials. Additionally, all out flavonoid substance in aqueous extracts of flowers of Campsis radicans were ۲.۳۸ ± ۰.۴ QE/g dry plant materials The synthesized nanoparticles also showed good antibacterial potential against gram-positive and gram- negative bacterial strains. Due to the presence of phenol and flavoonoides as antioxidant compounds in the extract of this plant. Its anticancer properties are recommended.

کلیدواژه ها:

Campsis radicans ، Silver nanoparticles ، Flavonoids ، Antibacterial activity ، Green nanoparticles