Effect of simulated microgravity condition on mouse myoblast (C۲C۱۲) cells growth

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 225

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شناسه ملی سند علمی:

BIOCONF21_0775

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

This study aimed to investigate the effect of simulated microgravity on the growth of mouse myoblast (C۲C۱۲) cell line. It has been believed that microgravity directly can modify the structure, function, and morphology of biosystems and numerous research have been performed to recognize these alterations. As a direct consequence of exposure to microgravity astronauts experience several physiological alterations, which can have serious medical implications when they return to Earth. The C۲C۱۲ cell line was purchased. The ۲D clinostat was applied for the simulation of the microgravity. Then, the morphological studies, acridine orange/propidium iodide (AO/PI) staining, and MTT cytotoxicity assay were utilized to determine any alternation in cells after ۴۸ h simulation microgravity exposure. However, after ۷۲ h exposure about ۴۰% of cell death occurred (P<۰.۰۵). The Acridine orange/PI staining confirmed this observation too. This finding could help astrobiologists to realize major health risks for astronaut crews and space travelers and reduce these harmful effects. Furthermore, our observations can open fascinating research lines in astrobiology, biophysics, and exobiology

نویسندگان

Narjes Rahmanian

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;

Azadeh Hekmat

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;

Zahra Hajebrahimi

Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran