Confirmation of effective immunotoxin constructs in breast cancer

Immunotoxins often referred to as "targeted therapies," are a combination protein consisting of a toxic moiety that is targeted to a specific deletion of target cells. The targeting part is generally a monoclonal antibody or genetically engineered antibody fragments. Antibody-based biologics are one of the well-known treatment strategies in cancer therapy. After modeling and simulating the desired immunotoxin in an aqueous medium and evaluating its binding affinity to the EGFR antigen, the immunotoxin was synthesized. In order to amplify and confirm the synthesized immunotoxin, the following steps were performed in vitro. The bacterial strain E.coli DH۵α is used to amplify plasmid DNA. Therefore, before starting the experimental steps, it is necessary to confirm the absence of any foreign plasmids in this bacterium. For this purpose, the linear culture of this strain was performed in the environment with and without antibiotics, which showed that the bacteria in the environment without antibiotics have very good growth, while in the environment with it, there is no growth, so it lacks The gene is resistance. Thus, new methods based on genes, cells, hormones, and bacteria have made it possible to understand the difference between normal and deformed cancer cells at the molecular level. Then, after determining and selecting the best structure, its synthesis, cloning, and approval were performed in laboratory conditions

کلیدواژه ها:

Cancer ، Targeted Therapy ، Cloning ، Combined Protein