Evaluation of the presence of Campylobacter Jejuni and Listeria Monocytogenes in water sources of Kermanshah city before chlorination based on comparison of culture and PCR

Despite abundant advances in water and wastewater treatment, waterborne diseases still threaten the health of the people of the world. Listeria Monocytogenes bacterium is a pathogen that causes listeriosis. This pathogen can also cause meningitis, poisonous sepsis and abortion in humans. One way of transmitting this microorganism is water and Food. Quick and accurate identification plays an important role in preventing infections. Also, due to the importance of Campylobacter Jejuni in water and food industries and causing infection, toxication and digestive problems in humans .identification of this bacterium can be an effective step in preventing water contamination with Campylobacter Jejuni. The aim present study was to identify Listeria Monocytogenes and Campylobacter Jejuni through culture and PCR and compare them in water supply of Kermanshah city. ۱۸ samples before chlorination, ۲ liters for PCR and ۲ liters for culture were collected from different water supplies in Kermanshah. DNA was extracted from standard Campylobacter Jejuni and Listeria Monocytogenes using DNG-Plus kit. PCR reaction was optimized using specific primers . After determining the specificity and PCR limit of detection, the collected water samples were examined and at the same time the samples were cultured and examined. From ۱۸ samples of water supply sources in Kermanshah by PCR, Campylobacter jejuni were isolated from all samples, and Lysteria Monocytogenes were isolated from۱۷ samples, and also ۴ cases of Campylobacter Jejuni and ۲ cases of Listeria Monocytogenes were isolated by culture method. The results show that PCR has a better performance than culture for detecting Listeria Monocytogenes and Campylobacter Jejuni.

کلیدواژه ها:

Meningitis ، Digestive problems ، Septicemia ، listeriosis