Antibacterial Activity of Shilajit

Pseudomonas aeruginosa and Staphylococcus aureus are the most important causes of nosocomial infections in a wide range of immunocompromised patients, including malignancies, cystic fibrosis, and others. Shilajit is a blackish-brown substance that forms next to boulders adjacent to underground oil reserves and is traditionally used to treat bone fractures. The aim of this study is investigating the effect of Shillajit on the growth of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Shilajit is prepared from Sardoyeh area of Jiroft city, Iran. Then the aqueous solution was prepared at the concentration used by the natives to heal the wound. The amount of ۱۰۰ cc of solution was dried completely at ۷۰ ° C for ۳۰ hours. ۳۰ mg of powder was dissolved in ۳ ml of sterile distilled water to prepare a stock solution. Concentrations of ۱۰۰%, ۷۵%, ۵۰% and ۲۵% of this solution were prepared and the antibacterial effects were assayed. The antibacterial activity was studied by disk diffusion method and measurement of the diameter of inhibition zone. The disk containing the extract was used as the sample and the disk containing the solvent was used as the negative control and the disk containing the antibiotic ampicillin as the positive control were placed on the culture medium containing the bacteria. The plates were placed in the incubator at۳۷ degrees temperature, for ۲۴ to ۴۸ hours. Then the growth inhibition zone diameter was measured by a millimeter ruler. The results showed that there was a significant difference between the diameter of the growth inhibition zone of the samples containing the extract with positive and negative controls (p <۰.۰۵). This study showed that shilajit had an inhibitory effect on the growth of these bacteria and could be a suitable alternative to antibacterial drugs of chemical origin.

کلیدواژه ها:

Shilajit ، Antibacterial activity Staphylococcus aureus ، Pseudomonas aeruginosa