The study of genotoxic and cytotoxic effects of the gum of Ferula gummosa on A-۳۷۵ cells

Melanoma is a malignant skin cancer with a high potential for metastasis that arises from melanin-producing cells. A-۳۷۵ cells, are proper model for the study of cancer drugs and inhibitors. Ferula gummosa grows in different parts of Iran. In the past, it used to reduce abdominal pain, to treat Rheumatism and diabetes, and also is used as laxative. F. gummosa has a milky white gum obtained from its root and bark. In this study, we investigated the cytotoxic effect of F. gummosa on A-۳۷۵ cells. After preparation of the gum of F.gummosa, A-۳۷۵ cells were treated with different concentrations of the gum for ۴۸ hours. The IC۵۰ value of the gum for A-۳۷۵ cells was calculated to be ۸ μg/ml. The DNA fragmentation assay showed that treatment of cells with lower concentrations of the gum also led to cell death and genomic DNA fragmentation. The results of wound healing assay showed that the gum inhibited the invasion of treated cells in concentration-dependent manner. The results of Micronucleus experiments showed that the gum of F. gummosa induced the formation of micronucleus and inhibited the cell proliferation in treated cells. Our results showed that, due to having different chemical compounds, the gum of F. gummosa can inhibit cancer cells proliferation.

کلیدواژه ها:

Ferula gummosa ، Cytotoxicity ، Genotoxicity ، Melanoma