Cytotoxicity assay of some amide derivatives using luminescent Vibrio isolated from the Caspian Sea

Today, given the extensive pollution of water, food, and environment by different pollutants, use of novel, fast, and efficient methods of detecting pollutants is being paid much attention. One of the methods of measuring the toxicity of the samples is to use luminescent bacteria for assessing toxicity based on bioluminescence inhibition. The unique characteristic of luminescent bacteria makes them suitable for measuring toxicity. The objective of this study was to measure the toxicity of amide compounds and their derivatives in foods by bioluminescence inhibition of luminescent Vibrio MM۱. The luminescence of the luminescent bacteria were assessed by a luminometer. To do this, one ml of some amide derivatives including acrylamide, tio-urea, and bis-acrylamide at ۱۰-۱-۱۰-۸ gL-۱ was added to one ml of bacterial growth medium for ۱۸-۲۴ h. The toxicity of amide derivatives was measured based on the luminescence reduction of Vibrio by using luminometer. To assess the toxicity, EC۵۰ parameter was measured. The results showed that the luminescence greatly decreased in the presence of amide derivatives. Bis-acrylamide at ۱۰-۱ gL-۱ had the greatest effect on the luminescence reduction as the Vibrio luminescence decreased from ۱۴×۱۰-۶ RLU to ۳.۵×۱۰-۶ RLU. Also, EC۵۰ values for acrylamide, bis-acryl and tio-urea were ۲.۶۳×۱۰-۴, ۱.۱۷×۱۰-۴, and ۲.۱۸×۱۰-۴ gL-۱, respectively. Thus, bis-acrylamide showed more toxicity than acrylamide and tio-urea. The results revealed that the luminescent bacterium Vibrio MM۱ was a good choice for examining the presence of amide compounds in food and wastewater.

کلیدواژه ها:

luminescent bacterium ، toxicity assey ، wastewater ، pollution