Synthesis and studying of Nano-Niosome formulation containing Thymus essential oil and its effectiveness in fungal infections

In this study, we optimized the Nano-Niosome formulation containing Thymus essential oil(TEO). The effect of the dose of this drug for the treatment of fungal infections was investigated. We aim is to reduce the dose, cytotoxicity and prevent fungal infections in cell culture and a suitable alternative to chemical drugs .In this study, we prepared Nano-Niosomes based on surfactant for proper attachment to the fungal wall and using the thin-film hydration method. using the UV-Spectrophotometric (evaluate of encapsulation and drug release ), DLS( determine the size), TEM (determine the morphology), FTIR spectroscopy( interaction between drugs and Nanocarrier) . The structure of Niosomes containing tween ۶۰: cholesterol: DPPC: PEG (ratio ۲۰:۳۰:۵۰:۳), was designed and developed. In the proposed formula: Rate of entrapment efficiency ۸۸.۲۰%, Niosomes-Thymus ۲۴h, ۵۴ ٪, size ۱۵۰ nm, dispersion index ۰.۲۲ and Zeta is (-۲۰.۵۶mv). FTIR spectral data showed that drugs and Nano-carriers did not react chemically with each other. Free drug molecules (Niosome and TEO) at a concentration of ۱۰۰ μg/ml caused the toxicity of cells, and the survival rate of the cells is, ۹۱.۵%, ۳۸% respectively. Whereas, the toxicity of encapsulated drugs at a concentration of ۲۰۰ μg/ml, was lower compared to free drugs, and the cell survival rates were, ۵۴.۸۸%. We were able to optimize Nano-carrier drug-delivery compounds based on surfactant and proportional to the structure of fungal cells, which has the ability to bind to the fungal cell wall. As a result, we can with this method treat or prevent fungal-infected cells.

کلیدواژه ها:

Nano-Niosome ، Encapsulation ، Thymus essential oil ، fungal cells