Cannabis sativa Extract suppresses the expression of iNOS in spinal cords of Experimental Autoimmune Encephalitis mice as a model of Human Multiple sclerosis

Experimental autoimmune encephalomyelitis (EAE) is an animal model of human CNS demyelinating disease, multiple sclerosis (MS). EAE is a complex condition in which the interaction between a variety of immunopathological mechanisms leads to an approximation of the pathological features of MS: demyelination ,inflammation, , axonal loss and gliosis. Inducible nitric oxide synthase (iNOS) is an enzyme that produces nitric oxide (NO) during the brain inflammatory conditions and is thought to contribute to the pathogenesis of multiple sclerosis. Cannabis sativa have been shown to pose potential therapeutic effects in different models of oxidative stress-induced neurodegenerative disorders.Herein, the potential effects of Cannabis sativa Extract (۱۰۰mg/kg/day i.p.), as an herbal antioxidant was evaluated on the mRNA expression iNOS using real-time RT-PCR, following the induction of EAE. Although the expression of iNOS was increased in EAE mice spinal cords, administration of Cannabis sativa Extract could significantly suppress its expression at day ۹ after induction of EAE, resulting in alleviation of overall inflammatory responses This indicates a potential therapeutic value of Cannabis sativa in MS patients and defines a new pharmacological tool for reducing the neuroinflammation and oxidative injury occurs in brains and spinal cords of the MS patients.

کلیدواژه ها:

Multiple sclerosis ، iNOS ، Cannabis sativa ، EAE