Evaluation of the antibacterial effects of diffrenet fractions from iranian scorpion venom Buthotus schach (BS), in – virto condition

Nowadays, the development of drug resistance in pathogenic bacteria to many antibiotics has an important and fundamental role in the lack of control and treatment of bacterial infections and is a major concern in human societies. Scorpion venom is rich in biologically active compounds and components that may be used in the development and discovery of new antibacterial drugs. So far, many compounds such as neurotoxins, salts, proteins and peptides with therapeutic properties have been identified in them that can quickly kill a wide range of bacteria. The aim of this study was to investigate the antibacterial properties of Hottentotta (Buthotus) schach. Scorpion venom was obtained by electrical stimulation and its peptides were purified by gel filtration chromatography. Then, the protein concentration was calculated with nanodrop and the quality of the poison was evaluated by SDS-PAGE electrophoresis. Dilutions ۱/۱ - ۱۰/۱ - ۱۰۰.۱ μg / ml Fractions were prepared from the initial concentration of ۱ ml / mg for plate well diffusion method and MIC based on CLSI ۲۰۱۹ protocol to determine the antimicrobial effect of the venom on bacteria. Gram-negative Escherichia coli ATCC ۲۵۹۲۲ and Pseudomonas aeruginosa ATCC ۹۰۲۷ and Gram-positive bacteria Staphylococcus aureus ATCC ۲۵۹۲۳. The results showed that F۱ and F۲ fractions of B. schach scorpion venom had a significant inhibitory effect on Gram-positive bacterium S. aureus and Gram-negative E. coli. This antimicrobial effect on Ps . aeruginosa was much weaker. The results of the hemolysis test also showed that at concentrations similar to MIC, the toxin had no hemolytic effect on fresh human blood. Therefore, suitable therapeutic indicators can be found in natural AMP of scorpion venom. They themselves are good candidates for the production of antimicrobials.

کلیدواژه ها:

schach scporpion ، Chromatography ، E. coli ، S. aureus ، Ps. Aeruginosa