Synthesis and evaluation of Chitosan Chloride/plasmid nanoparticles and evaluation of plasmid release from nanoparticles by electrophoresis and PCR

Chitosan chloride is a derivative of chitosan and, like chitosan, is a biodegradable polymer with no toxicity. Because of the weaker interaction with DNA, DNA release from chitosan chloride/DNA nanoparticles is more easily performed, today, chitosan chloride/DNA nanoparticles are of great interest to researchers and have been studied in this study. After adsorption of plasmid DNA (size ۳۶۳۳ bp) on the surface of chitosan chloride nanoparticles by simple complex method, the size and morphology of nanoparticles were examined by scanning electron microscopy (SEM). Agarose gel and UV rays were used to confirm the adsorption of plasmid on the surface of nanoparticles. The release of plasmid by treatment with the enzyme lysozyme and soluble phosphate-buffered saline (PBS) was investigated. PCR was also used to further evaluate the release. The results showed that the average size of nanoparticles is less than ۱۵۰ nm and the morphology of nanoparticles is spherical. Examination of agarose gel revealed that plasmid DNA was adsorbed on the surface of nanoparticles and after treatment, plasmid DNA was released, the results were confirmed by PCR. Because chitosan chloride/plasmid nanoparticles are easily synthesized, despite the protection of DNA, DNA release from them also occurs easily, they are cost-effective and suitable for therapeutic purposes.

کلیدواژه ها:

Simple complex ، release ، adsorption ، lysozyme ، biodegradable