The effect of donepezil on CyP۱۹a۱ gene expression in female Wistar rats with polycystic ovary syndrome

Evidence suggests that the role of neurotransmitters in polycystic ovary syndrome (PCOS) is not yet fully understood. However, it has been shown that in PCOS conditions the level of the neurotransmitter acetylcholine (ACh) decreases. Previous studies have shown that the growth and differentiation of ovarian follicles may be regulated by ACh. The aim of this study was to evaluate the expression of CyP۱۹a۱ gene after injection of donepezil (A type of acetylcholinesterase inhibitor) in female wistar rats with PCOS. For this study, ۲۵ female wistar rats (۳ groups of ۵ with PCOS and ۲ groups of ۵ negative and positive controls) were used. Induction of PCOS in rats (۱۶۰-۲۰۰ gr) was induced by intramuscular injection of estradiol valerate (۴ mg per ۱۰۰۰ gr body weight, Abu Reihan Co., Iran). In order to evaluate the effect of donepezil on the steroidogenesis pathway of ovarian follicles, Intraperitoneal injection of this drug was performed in ۳ groups with PCOS at doses of ۲, ۴ and ۶ 𝜇𝑚𝑜𝑙/𝐾𝑔. The effect of donepezil was evaluated by ovarian extraction and CyP۱۹a۱ gene expression. The results of CyP۱۹a۱ gene expression show that injection of donepezil increases CyP۱۹a۱ (p < ۰/۰۵) gene expression in PCOS groups compared to the control group. According to the results of this study, it seems that the improvement of PCOS conditions in the treatment with donepezil is due to the increased expression of CyP۱۹a۱ gene.

کلیدواژه ها:

Acetylcholine ، Aromatase ، Steroidogenesis