Investigation of the L- Carnitine effect on angiogenesis and granoluza cell proliferation in transplanted mouse ovarian tissue

The aim of this study was to investigate the role of L- Carnitine (LC) as an antioxidant on angiogenesis and follicular cell proliferative status in transplanted mouse ovarian tissue. Ovarian tissue transplantation is an emerging technology for fertility preservation. A major obstacle in survival of the ovarian implants is vascular failure which leads to tissue necrosis. NMRI mice were divided into four groups. Control (non-grafted), Transplant (autograft without treatment), Saline group (autograft+ saline), LC group (autograft+ LC). ۶- weeks- old mice were ovariectomized and left ovaries were transplanted into the back muscle tissue. LC (۲۰۰ mg/Kg) was injected intraperitoneally one day before surgical operation and repeated until one week after grafting. ۳ weeks later, ovarian grafts were recovered and were immunohistochemically assessed. Anti- mouse CD۳۱ immunohistochemical staining (staining epithelial cells of new blood vessels) was performed in ۶- μm thick sections to evaluate neo-vascularization of the grafted tissues. The proliferative follicular cells was assessed by nuclear antigen Ki-۶۷ immunostaining. We quantified and compared neo-vascularization and also proliferative index in all groups. Our results indicated that the expressions of tested proteins in control group were higher than transplanted groups, but their expressions had no statistically differences between transplanted groups. Our results showed that LC alone didn’t show significant effect on angiogenesis and proliferative index in transplanted tissue. These results may be for lasting ۳ weeks after grafting and this study should perform in first week after transplantation. It seems that usage of LC in combination with a scaffold or growth factors could improve autotransplantation results.

کلیدواژه ها:

Ovarian transplantation ، CD۳۱ ، Ki-۶۷