Effect of inhibition of D۲ dopamine receptors on offspring’s fertility of intact male rats

Dopamine is one of the most abundant central and peripheral neurotransmitters that its D۲ receptor has been found in testicular and ovarian tissues. Probably, using the drugs that affect the reproductive system Influence the physiological processes of the offspring of the parents under treatment. The aim of this study was to investigate the effect of dopamine D۲ receptors (D۲R) inhibition on the fertility of offspring of intact male rats. Twenty adult male Wistar rats weighing ۱۹۰±۱۰ were divided into two groups including saline and sulpiride (receptor antagonist of D۲ dopamine) (۴ mg/kg bw, ip) received. After ۱۴ days treatment, each male rat mated with three adult female rats; female offspring of these rats were raised to normal under puberty; then body and ovaries weight, and the fertility of offspring were assessed using IVF. The mean body and ovaries weight, as well as the percentage of egg cells, biceps, blastocyst embryos, and fetuses hatched after IVF were significantly reduced in offspring of male rat under treatment with sulpiride compared with saline control group (p<۰.۰۵). Also, following the inhibition of dopamine D۲ receptors in the parent, the percentage of discontinued embryos resulting from IVF of female offspring showed a significant decrease (p<۰.۰۵). Inhibition of dopamine D۲ receptors in male parents reduced the fertility of female offspring.

کلیدواژه ها:

IVF ، Sulpiride ، Fertility ، Dopamine