Study of the Effect of Genistein on the Renal Function in the Ischemic Acute kidney Injury in Male Rat

Background and Aim: It is well known that there are gender differences between susceptibility to acute kidney injury (AKI) and ۱۷ β-estradiol (E۲-β) protects against the post-ischemic AKI. Genistein is a phytoestrogen which can be an agonist of estrogen β receptor. The goal of this study was to determine whether genistein has a protective role against renal ischemia/reperfusion (IR)-induced functional disorders of the kidney in male rats. Methods: Rats under anesthesia by an intraperitoneal (i.p.) injection with ketamine and xylazine were placed on a warming surgical table. Following incision in the midline of abdomen, artery and vein of both kidneys were carefully separated from each other The animals were dedicated to five groups (n=۷): sham, G + sham (genistein, ۱۵ mg/kg in ۱ ml ۱% DMSO, i.p.), DMSO + sham (۱ ml ۱% DMSO, i.p.), I/R (۴۵ min bilateral renal ischemia followed by ۲۴ h reperfusion), G + I/R (genistein, ۱۵ mg/kg in ۱ ml ۱% DMSO, i.p. ۳۰ min before the induction of ischemia as well as ۱ h after the induction of ischemia). Then, the rats were put in metabolic cages for ۲۴ hours, and subsequently, urine, blood and tissue samples of the kidney were collected. Results: Compared to sham, sham+G and sham+DMSO groups, IRI ended in kidney dysfunction, as revealed by decreased creatinine clearance (CCr), increased fractional excretion of sodium (FENa) and potassium (FEK), as well as decreased effective free water reabsorption (effTcH۲O). This was happened with an increase of malondialdehyde levels in renal tissue. Genistein administration decreased all the changes. Conclusion: Genistein apparently protects against renal I/R-induced injuries of the kidney via increasing renal blood flow and decreasing lipid peroxidation in kidney tissue.

کلیدواژه ها:

Creatinine ، Clearance ، Fractional excretion ، Ischemia/reperfusion ، Lipid peroxidation