Consequences of Simulated Microgravity in Biosystems: Structural Effects and Cellular morphology

Enhancements in technology have offered extraordinary opportunities for the human to travel more rapidly on or near the surface of the Earth. The primary goals of space travel are the search for life, planetary exploration, and more significantly safe return to Earth. Humans on Earth are adapted to the constant gravitational force (۹.۸ m/S۲). Nevertheless, in space, gravity is much weaker than on Earth which is known as microgravity. Presently, investigations on the growth and development of cells as well as bio-macromolecules structure exposed to microgravity, as biophysical force, is a hot topic in cell biology and astronauts’ health. Consequently, we first investigate the probable impacts of simulated microgravity on the structure of human serum albumin (HAS), histone H۳, and DNA by multiple spectroscopic techniques. Subsequently, we analyzed the effects of simulated microgravity on the growth of MCF۷ and T۴۷D breast cancer cell lines. Our results exposed that the structure of HAS, histone H۳, and DNA subjected to simulated microgravity changed significantly. Furthermore, our results showed that microgravity simulation did not have a remarkable effect on the viability of cells, but cellswere grouped and linked to each other making multicellular spheroids. The findings achieved from this investigation can open fascinating research lines in biophysics, astrobiology, and biology and can be utilized to enhance survivability and life quality for space travelers.

کلیدواژه ها:

Astrobiology ، Cell viability assay ، DNA structure ، Protein structure ، Microgravity