Human Papilloma Virus Type ۱۶ in Epithelial Ovarian Cancer

Background and Aim : Background: Ovarian carcinoma is the most common malignancy of women and the cancer with a ۱۵–۵۰% in the world. Human Papilloma Virus (HPV) is considered factors in cervical and ovarian cancer (OCa) and are related with squamous cell carcinoma in cervical region. The effect of fixed infection maybe causing to chronic inflammation, in the cancer of ovaries has received very rare attention, although a background of pelvic inflammatory disease (PID) is in a case-control study associate to higher risk for ovarian carcinoma. HPV-۱۶ is one types of HPV and its the most common and important cause of cervical carcinoma in the developed world. Objectives: The aim of this investigate was evaluate incidence of HPV-۱۶ in patients with OCa who referred to Imam Hossein Hospital of Shahid Beheshti University of Medical Sciences, Tehran-Iran.Methods : Materials and Methods: In this case-control study that was conducted from May ۲۰۱۵ to October ۲۰۱۵ in Tehran, ۱۴۰ samples were studied which obtained from patients with OCa. After obtaining samples from OCa tissue by the pathologist, for extraction DNA, samples were transferred to the laboratory of university. DNA was extracted with Kit (intron Biotechnology Co. Korea) according to manufacturer instructions. To confirm the presence of HPV-۱۶ in samples of OCa, specific primers for the L۱ gene of HPV, were designed and used standard PCR method for detection of HPV. PCR product was sequenced to confirm the presence of HPV-۱۶.Results : Results: Out of ۱۴۰ samples of OCa,۷۰ (۵۰%) samples were malignant cancer and ۷۰ (۵۰%) were benign cancer as control group. From ۷۰ malignant samples ۲۵(۳۶.۰%) were HPV-۱۶ positive. ۲.۸% of the tissue samples of control group were positive for HPV-۱۶.Conclusion : Conclusion: This results shows a feasible role of HPV-۱۶ in the carcinogenesis of OCa. According to this results infection with HPV may play a relative role in the spread of OCa or it could comfort its development.

کلیدواژه ها:

HPV ، Ovarian Cancer ، Malignancy ، PCR