Investigation of Toll-like receptor related genes in co-infection of IBV and APEC in the chicken trachea using RNA-Seq

Background and Aim : Infection with Infectious bronchitis virus (IBV) and avian pathogenic E.coli (APEC) is an important respiratory infection in Worldwide. Dendritic cells (DCs), macrophages, lymphocytes, endothelial cells, and fibroblasts present PRRs such as Toll-like receptors (TLRs) that can recognize infectious agents through them that the identification of them is important for controlling programs. The aim of this study was to investigate on Toll-like receptor related genes in the trachea tissue of infected (IBV variant ۲, and with APEC serotype O۷۸: K۸۰) SPF chickens group compare to the control group.Methods : A number of Forty SPF chickens were divided into two treatment and control groups. Differential transcriptional profile in the trachea tissue of infected SPF chickens group compares to the control group in the early stage of infection by Illumina RNA-seq technique paired-end and strand-specific sequencing. DEGs of transcriptome profiling of the trachea from the infected group were identified. Gene ontology category, KEGG pathway, and STRING analysis were used to identify relationships among differentially expressed genes.Results : All Toll-like receptor genes in our study were up-regulated. The number of twenty-three Toll-like receptor genes was identified consist of CD۸۰, FADD, IFNAR۱, IFNAR۲, IL۸L۱, LY۹۶, MAP۳K۷, MAPK۱, MAPK۱۴, MYD۸۸, NFKB۱, PIK۳CA, PIK۳CB, PIK۳CD, PIK۳R۲, RIPK۱, STAT۱, TLR۲-۲, TLR۳, TLR۴, TLR۷, TOLLIP, TRAF۶.Conclusion : Among them, the most important factors in identifying bacterial and viral products such as TLR۲, TLR۴ (recognize peptidoglycan and LPS), TLR۳ and TLR۷ (recognize viral products) was observed.

کلیدواژه ها:

TLRs ، IBV ، APEC ، RNA-seq