Genotyping of environmental Helicobacter pylori strains in Kurdistan water sources

Background and Aim : Epidemiological studies have shown an association between the prevalence of H. pylori infection and water hygiene levels. In developing countries such as Iran, contaminated Water is considered to be one of the main sources of human pollution to H. pylori. The aim of this study was to determine the frequency of H. pylori and its virulence genes in Sanandaj and Marivan water sources samples.Methods : In this study, ۱۰۰ samples of water collected from different areas of Sanandaj and Marivan were studied. After filtration of water samples, the genome of potential organisms in the samples was extracted using PowerSoil® DNA Isolation Kit (Qiagen). H. pylori contamination was determined by using PCR and specific primers for ۱۶S rDNA gene.Results : In this study, two samples were positive for H. pylori. In these two samples, oipA (۱۰۰%), sabA(۵۰%) genes were determined.Conclusion : In this study, the similarity of the isolated genotypes with the genotype of the clinical strains suggests that these strains may be cloned in the human stomach if hygiene is not observed. Both strains lacked the pathogenic and important gene cagA. Therefore, it can be concluded that these suspensions were less pathogenic.

کلیدواژه ها:

Helicobacter pylori ، Water sources ، Genotyping ، Virulence genes