Probiotic bacteria induces apoptosis of Gastric cancer through signaling pathways in H. pylori

Background and Aim : Gastric cancer (GC) is considered as the second prevalent agent that leads to death a high population of humans around the world. This type of cancer is generally induced by Helicobacter pylori which could be colonized the gastric mucosa of at least half of the population worldwide. Today, triple antibiotic therapy is routinely utilized for controlling the H. pylori-induced infection. However, this strategy is unsuccessful because of some obstacles such as occurring point mutations in the H. pylori genome resulting in resistance to the antibiotics. Recently, it is showed that different probiotics have strong anti-cancer effects in which they are capable of inhibiting H. pylori by both immunological and non-immunological mechanisms. Here we tried to find some anti-cancer impacts of the probiotic bacterium L. plantarum on the gastric cells (AGS cell line).Methods : The anti-cancer effects of the conditioned media of the locally isolated L. plantarum on the AGS cells were evaluated by different analyses such as flow cytometry, DNA ladder assay, DAPI staining and, RT-PCR.Results : our results showed that the conditioned media of L. plantarum can inhibit both H. pylori and AGS cells through up/down regulation of PTEN, Bax, TRL۴, and AKT genes.Conclusion : Different reports confirmed the anti-cancer effects of several strains of probiotics especially lactobacilli. Accordingly, it seems the probiotics could be considered as at least an assistant treatment for the many types of cancers.

کلیدواژه ها:

Gastric cancer ، Helicobacter pylori ، Lactobacillus plantarum ، Apoptosis ، PTEN ، AKT