Study of some of the most important antibiotic resistance genes encoding in Acinetobacter baumannii isolated from raw milk in Karaj city

Background and Aim : Acinetobacter baumannii is one of the causes of nosocomial infections resistant to antibiotic treatments. The presence of some genes encoding resistance is considered as one of the mechanisms of antibiotic resistance in Acinetobacter baumannii strains. The present study was performed to determine the frequency of some genes encoding antibiotic resistance in Acinetobacter baumannii strains isolated from raw milk collected from Karaj city-Alborz.Methods : A total of ۵ isolates of Acinetobacter baumannii were isolated from raw milk samples (that bought from traditional dairy shops in Karaj city-Alborz). Isolated strains were first confirmed by microbial culture and biochemical tests of IMVIC, urease, TSI, OF, MRVP, SIM, catalase and oxidase and growth evaluation at ۳۷ and ۴۲ ° C. Then DNA samples were extracted from Acinetobacter baumannii isolates and the presence of some genes encoding antibiotic resistance(tetA, aac (۳) -IV, CITM, baSHV and cat۱) was evaluated using PCR test.Results : The frequency of genes encoding tetA, aac (۳) -IV, CITM, baSHV and cat۱ antibiotic resistance in Acinetobacter baumannii isolates was ۸۰%, ۶۰%, ۸۰%, ۴۰% and ۲۰%, respectively.Conclusion : Prescribing antibiotics according to international guidelines and using simple and inexpensive methods such as the release of antibiotic discs can prevent severe antibiotic resistance in Acinetobacter baumannii strains.

کلیدواژه ها:

Acinetobacter baumannii ، raw milk ، genes encoding antibiotic resistance ، Karaj city