Investigating bacterial sources for vitamin K۲ production

Background and Aim : Vitamin K۲ is one of the three types of vitamin K, which produced by bacteria. It has an important role in bone and cardiovascular mineralization and prevents osteoporosis and cardiovascular disease that are of the major matters in public health concern. This vitamin is rare in dietary uptake. Thus, finding high vitamin production in bacterial strains can be valuableMethods : Ten soil samples from different regions of Iran includes Golestan, Mazandaran, Markazi, and Tehran provinces were collected. These include forest, garden, farm, forest park, and red soil fields during April and May ۲۰۱۹. Bacillus strains were isolated and investigated for vitamin K۲ production through liquid state fermentation in specific media contain soy peptone, yeast extract, glycerol, and K۲HPO۴. The vitamin concentration measured in ۲۴۸nm UV-spectroscopy following extraction by n-hexane: ۲-propanol. High vitamin producing strains were biochemically characterized.Results : From ۹۷ isolated strains, ۲۰ strains have higher absorbance value in comparison with a standard calibration curve. According to the biochemical characteristics, high vitamin producer isolates were Bacillus subtilis, Bacillus cereus, and closely related bacteria.Conclusion : Isolating high vitamin K۲ producer strains is a global matter and soil is widely used as a major source of bacillus species that are candidates for high vitamin K۲ producing strains.

کلیدواژه ها:

Vitamin K ، Bacterial sources ، Bacillus