Investigation of synergistic effect of biosynthesised silver nanoparticles by chlorella vulgaris and curcumin against clinical isolates of Pseudomonas aesuginosa and Staphylococcus aureus

Background and Aim : this study was done to evalute the synergistic effect of bio-silver nanoparticles synthesized by the chlorella algae and curcumin against clinical isolates. Pseudomonas aeruginosa and Staphylococcus aureus.Methods : Silver nanoparticles were synthesized by the chlorella algae biologically. Then, spectrophotometers and XRDs were used to characterize the synthesis of silver nanoparticles. Then, the antimicrobial effect of silver nanoparticles and curcumin were separated and combined. The wells were examined carefully and finally their MIC and MBC were evaluated.Results : The antibacterial effect of nano-silver Showed that it had more inhibitory effect on Pseudomonas aeruginosa isolates. These results obtained from the antibacterial effect of curcumin showed that the extract had no inhibitory effect on Pseudomonas aeruginosa isolates while ethanolic curcumin extract had more inhibitory effect than the aqueous extract on Staphylococcus aureus isolates. It had more anti bacterial effect on Staphylococcus aureus isolates than Pseudomonas aeruginosa .. The synergy of bio-nano-silver and nano-curcumin showed that this compound had more inhibitory effect on Pseudomonas aeruginosa isolates than Staphylococcus aureus.Conclusion : The results of this study showed that bio-silver in combination with nano-curcumin had synergistic effect on growth inhibition of Pseudomonas aeruginosa and Staphylococcus aureus and had more inhibitory effect on Pseudomonas aeruginosa isolates. Combination therapy increases the effectiveness of each in treating infections caused by Pseudomonas aeruginosa and Staphylococcus aureus. Further studies are needed to elucidate the toxicity of these particles.

کلیدواژه ها:

Silver Nanoparticle ، Antimicrobial Effect ، ، Curcumin ، Synergy